Lyophilized peptides are supplied as freeze-dried powder to maximize stability during storage and shipping. Before use in laboratory research, they must be reconstituted by adding a suitable solvent. This guide covers the standard reconstitution process used in research settings.
What is Lyophilization?
Lyophilization, or freeze-drying, is a preservation process that removes water from a compound under low temperature and pressure conditions. The result is a stable powder that resists degradation and can be stored long-term at -20°C. All peptides supplied by Alpha Tides PNW are provided in lyophilized form.
What Solvent to Use
The most commonly used solvent for peptide reconstitution in research settings is bacteriostatic water — sterile water containing 0.9% benzyl alcohol, which inhibits microbial growth and extends the usable life of the reconstituted solution.
Other solvents used in research include:
- Sterile water for injection
- Acetic acid (0.1%) for peptides that are difficult to dissolve
- DMSO for highly hydrophobic peptides
Bacteriostatic water is the standard choice for most research peptides including CJC-1295, Ipamorelin, Tesamorelin, and GLP-3(R).
Reconstitution Process
The standard laboratory reconstitution process involves the following steps:
Allow the lyophilized vial to reach room temperature before opening to prevent moisture condensation on the powder. Using a sterile syringe, slowly inject the desired volume of bacteriostatic water into the vial, directing the solvent toward the glass wall rather than directly onto the powder. Gently swirl the vial — do not shake — until the powder is fully dissolved. The resulting solution should be clear with no visible particulates.
Storage of Reconstituted Peptides
Reconstituted peptide solutions should be stored at 2-8°C (standard refrigerator temperature) and used within 28-30 days. Avoid repeated freeze-thaw cycles as these can degrade the compound. Keep solutions away from light and heat.
Lyophilized powder that has not been reconstituted should remain stored at -20°C until needed.
Purity and COA Documentation
Alpha Tides PNW provides Certificate of Analysis documentation for every batch of peptides supplied. COA documents confirm compound identity and purity verification by independent third-party laboratory analysis. Researchers can request COA documentation for any order.
Research Use Only
All peptides supplied by Alpha Tides PNW are intended strictly for laboratory and in vitro research purposes. They are not for human or animal consumption and have not been evaluated or approved by the FDA.